1、首先準備好受血者和獻血者的血樣。

2、配好用受血者血清配成的2%的紅細胞懸液；用獻血者血清配成的2%紅細胞懸液。

3、主側(cè)加受血者血清0.5ml，獻血者血清配成的2%紅細胞懸液0.25ml.

4、次側(cè)加獻血者血清0.5ml，受血者血清配成的2%的紅細胞懸液0.25ml.

5、37度水浴箱孵育1小時，后觀察有無凝集或溶血。

如有配血禁忌，則應(yīng)查原因。